fitcconjugated mouse anti chicken cd4  (Bio-Rad)

Journal: Poultry Science

Article Title: Baseline immune profiles of local chicken breeds: linking biodiversity, animal health, and vaccination response

doi: 10.1016/j.psj.2025.105565

Figure Lengend Snippet: Composition of αβ TCR+ T cell subpopulations in PBMC of naïve chickens. ( A ) Flow cytometry gating scheme for analysis of αβ TCR+ T cells. ( B ) Kinetics of αβ TCR+ T cell subtypes in PBMC of naïve adult chickens from the 6th until the 60th week of age. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing CD25. Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). TCR = T cell receptor, PBMC = Peripheral Blood Mononuclear Cells, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher.

Article Snippet: Chicken CD4 , 2-35 , Pure , serotec , 1:1000.

Techniques: Flow Cytometry, Expressing

Journal: Poultry Science

Article Title: Baseline immune profiles of local chicken breeds: linking biodiversity, animal health, and vaccination response

doi: 10.1016/j.psj.2025.105565

Figure Lengend Snippet: Composition of T cell subpopulations in PBMC of naïve 35-week-old laying hens at peak performance, comparing local breeds and commercial lines. ( A ) γδ TCR+ T cell subtypes. Left figure: proportion of all γδ TCR expressing T cells among all CD3+ T cells and right figure: proportion of CD8+ γδ T cells. ( B ) αβ TCR+ T cell subtypes. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β, lower left: cells expressing CD4 among all αβ TCR expressing cells and lower right: proportion of CD4+ cells expressing CD25. Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). PBMC = Peripheral Blood Mononuclear Cells, TCR = T cell receptor, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher, WR = White Rock, RG = Ranger.

Article Snippet: Chicken CD4 , 2-35 , Pure , serotec , 1:1000.

Techniques: Expressing

Journal: Poultry Science

Article Title: Baseline immune profiles of local chicken breeds: linking biodiversity, animal health, and vaccination response

doi: 10.1016/j.psj.2025.105565

Figure Lengend Snippet: Composition of T cell subpopulations in blood, spleen and lung of day-old chicks (DOC). ( A ) γδ TCR+ T cell subtypes. Left figure: proportion of all γδ TCR expressing T cells among all CD3+ T cells and right figure: proportion of CD8+ γδ T cells. ( B ) αβTCR+ T cell subtypes. Upper left: cells expressing CD8α among all αβ TCR expressing cells, upper right: proportion of CD8α+ cells expressing CD8β and lower left: cells expressing CD4 among all αβ TCR expressing cells. Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***), and P ≤ 0.0001 (****). TCR = T cell receptor, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher.

Article Snippet: Chicken CD4 , 2-35 , Pure , serotec , 1:1000.

Techniques: Expressing

Journal: Poultry Science

Article Title: Baseline immune profiles of local chicken breeds: linking biodiversity, animal health, and vaccination response

doi: 10.1016/j.psj.2025.105565

Figure Lengend Snippet: Immune responses to Newcastle Disease Virus vaccination after experimental prime or prime/boost immunization . ( A ) Antigen specific cellular response after viral re-stimulation in vitro 28 days after boost vaccination. A 1:1 mixture of PBMC and splenocytes were re-stimulated with vaccine strain NDV clone 30 (circular symbols) or with velogenic strain NDV NR81/18 (triangular symbols). Empty symbols belong to prime group, filled symbols to boost group. Left figure: proportion of proliferated γδ TCR expressing T cells (among CD3+ T cells), middle figure: proportion of proliferated cells expressing CD4 (among αβ TCR expressing cells) and right figure: proportion of proliferated cells expressing CD8α (among αβ TCR expressing cells). ( B ) Antibody response ex vivo in course of the trial. Dashed bars belong to prime group, filled bars to boost group. Data are presented as geometric mean with 95 % confidence interval. ( C ) Oropharyngeal virus shedding. Dashed bars belong to prime group. Significance levels were indicated as follows: P ≤ 0.05 (*), P ≤ 0.01 (**), and P ≤ 0.001 (***). PBMC = Peripheral Blood Mononuclear Cells, Δ prol. cells = Δ proliferated cells (stimulated – unstimulated control), TCR = T cell receptor, ALT = Altsteirer, BIE = Bielefelder, RAM = Ramelsloher.

Article Snippet: Chicken CD4 , 2-35 , Pure , serotec , 1:1000.

Techniques: Virus, In Vitro, Expressing, Ex Vivo, Control

Journal: Frontiers in Veterinary Science

Article Title: Immune cell profile and metabolic preference following intramuscular lipopolysaccharide injection of highly inbred and advanced intercross genetic lines

doi: 10.3389/fvets.2025.1592021

Figure Lengend Snippet: Percentages of (A) Monocyte/Macrophage + , (B) total CD3 + , (C) CD3 + CD1.1 + , (D) CD3 + CD4 + , and (E) CD3 + CD8α + cells isolated from peripheral blood mononuclear cells of genetic line (Ghs, Line-8, Sp-21.1, and AIL-F) ± 1 mg/kg intramuscular LPS injection at baseline, 6 hpi, and 24 hpi. Data represents the mean ± SEM ( n = 10 birds/genetic line at baseline and n = 5/treatment of each genetic line at 6 hpi and 24 hpi). Note that the y-axis is larger in panels (B-E) . Different letter superscripts denote significant differences within a timepoint p ≤ 0.05.

Article Snippet: The flow cytometry panel included the following antibodies to detect innate immune cell and T lymphocyte populations: PE anti-chicken monocyte/macrophage (clone KUL01; mouse IgG1κ), Pacific BlueTM anti-chicken CD3 (clone CT-3; mouse IgG1κ), FITC anti-chicken CD1.1 (clone CB3; mouse IgG1κ), PE/CY7 anti-chicken CD4 (clone CT-4; mouse IgG1κ), Alexa Fluor ® 700 anti-chicken CD8α (clone CT-8; mouse IgG1κ; Southern Biotech, Birmingham, AL).

Techniques: Isolation, Injection